[Principle]
The feline panleukopenia virus (FPV) antigen rapid test card is a chromatographic immunoassay for the qualitative detection of FPV in samples.When a sample from the stool is added to the sample well on the card, it can move along the membrane with colloidal gold-conjugated potential anti-FPV monoclonal antibodies. If FPV antigens are present in the sample, they can bind to antibodies immobilized on the test line and a burgundy line appears within a few minutes. If there is no FPV antigen in the sample, no color reaction will occur.
[Kit composition]
No. | Name | 20T / box |
1. | test cards (including straws) | 20 |
2. | Sample dilution | 20bottles |
3. | Cotton swab | 20 sticks |
4. | instruction manual | 1 |
[Storage and expiry date] Store in a cool and dry place (2-30 ° C), valid for 24 months.
[Sample preparation]
After soaking the cotton swab with normal saline, swab the feces, rectum, vomit, and saliva. Immediately insert the cotton swab into the sample tube containing the diluent. Stir the cotton swab thoroughly until all the samples on the cotton swab are dissolved in the diluent. Place the cotton swab in the tube. The wall is squeezed out and discarded, and the sample solution is left to stand.
Note:
Excessive stool samples may lead to erroneous results (such as the occurrence of black bands, etc.), and can be diluted with the sample dilution solution before testing.
【experiment method】
1. Tear off the aluminum foil packaging bag of the test card, remove the test card, and place it on a flat, clean surface.
2. Pipette the prepared clear sample supernatant with a matching pipette, and slowly add 2-3 drops (about 60ul) into the sample well vertically and slowly.
3. Leave at room temperature for 5-10 minutes to judge the results. Results longer than 30 minutes are invalid.
